What does this process enable?
This may be a bit beyond what you need to know in terms of general molecular cloning in your exams etc. To keep focused you can watch the video below to get an idea of what basic molecular cloning looks like.
Gateway cloning is a very efficient more advanced form of this that uses different genetic exchange mechanism.
Gateway cloning is a very efficient more advanced form of this that uses different genetic exchange mechanism.
The gateway cloning technology can be used to clone or sub-clone (very different by the way) one or more DNA sequences of interest into multiple vectors (plasmids). This is done using specific site recombination. These vectors can then be transformed into hosts such as E.coli and then transfected into cell lines such as MEF’s. This overall can enable the analysis of the functionality of the gene of interest and elements of the protein expressed.
In order to form these transgenic hosts, processes such as Heat shock transformation into competent E.coli cells is required to enable the plasmids more readily into the bacterial cells. The amplification and selection process of these transformed bacterial cells enables the amplification of plasmids with the desired gene. This can be followed on by processes such as the Mini-Prep or Maxi –prep that would then enable the extraction of the successfully cloned or sub-cloned DNA for transfection into various cell lines. Again In doing this, the analysis of the functionality of the gene and the protein can be achieved.
In order to form these transgenic hosts, processes such as Heat shock transformation into competent E.coli cells is required to enable the plasmids more readily into the bacterial cells. The amplification and selection process of these transformed bacterial cells enables the amplification of plasmids with the desired gene. This can be followed on by processes such as the Mini-Prep or Maxi –prep that would then enable the extraction of the successfully cloned or sub-cloned DNA for transfection into various cell lines. Again In doing this, the analysis of the functionality of the gene and the protein can be achieved.