One of the big changes when you come to a top research university like Exeter is that there a loads of practicals that take place over the course of the year. You'll need to get to grips with how to behave in a real laboratory environment and the Exon Bloggers are here to help!
Pipetting
During our very first week of university, we were taught several key laboratory techniques. These techniques are used in almost every practical session. It is really useful for scientists carrying out experiments. Key laboratory techniques include aseptic techniques, microscopy, pipetting skills as well as streak and spread plating. I am going to be explaining the principles and application of pipetting. There are various kinds of pipettes ranging from one microlitre to one millilitre.
To pipette a liquid, first set the pipette to the amount you want. Then add on a disposable tip using aseptic techniques. After this, press down on the push-button to the first stop and place the disposable tip into the liquid to be pipetted. Release the push-button slowly. This pulls up the exact amount of liquid required eliminating any air being taken up. This can then be transferred into another beaker or container by pressing down the push-button to the last stop. This empties the disposable tip completely of any liquid. The disposable tip can then be disposed of.
Pipetting skills are very important because it is one of the key skills tested in most practicals in titration.
Pipetting is useful when measuring small amounts of chemicals.
To pipette a liquid, first set the pipette to the amount you want. Then add on a disposable tip using aseptic techniques. After this, press down on the push-button to the first stop and place the disposable tip into the liquid to be pipetted. Release the push-button slowly. This pulls up the exact amount of liquid required eliminating any air being taken up. This can then be transferred into another beaker or container by pressing down the push-button to the last stop. This empties the disposable tip completely of any liquid. The disposable tip can then be disposed of.
Pipetting skills are very important because it is one of the key skills tested in most practicals in titration.
Pipetting is useful when measuring small amounts of chemicals.